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KMID : 0545119970070060402
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Journal of Microbiology and Biotechnology
1997 Volume.7 No. 6 p.402 ~ p.408
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A Peptide Antibiotic AMRSA1 Active against Multidrug-resistant Staphylococcus aureus Produced by Streptomyces sp. HW-003
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Lee, Hyean Woo
Choi, Jong Whan/Kim, Hyun Won/Han, Dong Pyou/Shin, Woon Seob/Yi, Dong Heui
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Abstract
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The antibiotic-producing strain HW-003 was screened from soil and found to be effective against the multidrug-resistant Staphylococcus aureus. The spore chain of HW-003 was retinaculiaperti, and the spore surface was spiny. Strain HW-003 has a LL-diaminopimelic acid isoform in the cell wall. The aerial mass color of the strain was gray, and the reverse side was yellow-brown. The strain produced melanin, but did not produce soluble pigments. According io the Taxon program, HW-003 showed best match with Streptomyces cyaneus. Antibiotic production reached a maximum after 72-h cultivation. The antibiotic was purified with silica gel column chromatography, octadecylsilyl column chromatography, and HPLC. The purified antibiotic, AMRSA1. showed strong inhibitory activity against multidrug-resistant Staphylococcus aureus and gram-positive bacteria. The molecular weight of AMRSA1 was about 1,100. AMRSA1 was a peptide antibiotic containing alanine and serine.
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